Young individuals are always male. A subset of individuals from all groups above were maintained until hatching, then sexed Table 1. To determine whether this treatment alters SF-1 expression as well, we spotted eggs incubating at 26 C with E 2 at stage 17 and assayed four subsequent developmental stages for SF-1 message.
The turtles were incubated at temperatures that produce solely males, both sexes, and solely females. A series of stages in the embryonic development of Chelydra serpentina.
Blots were hybridized overnight at 43 C, then washed for 40 min with one solution change at each of three stringencies: 0. Cite this Page Gilbert SF. Namespaces Article Talk. Wennstrom KL , Crews D Effect of long-term castration and long-term androgen treatment on sexually dimorphic estrogen-inducible progesterone receptor mRNA levels in the ventromedial hypothalamus of whiptail lizards.